Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe₂O₃ nano-sized particles and assessment of their effects on glutamate transport

• Tatiana Borisova,
• Natalia Krisanova,
• Arsenii Borуsov,
• Roman Sivko,
• Ludmila Ostapchenko,
• Michal Babic and
• Daniel Horak

Beilstein J. Nanotechnol. 2014, 5, 778–788, doi:10.3762/bjnano.5.90

• uptake and release; manipulation by an external magnetic field; D-mannose; membrane potential; nanoparticles; rat brain nerve terminals; synaptic vesicle acidification; Introduction Nanoparticles have great biotechnological potential opening a wide range of new applications. Properties of nanomaterials

• nanoparticles did not influence the fluorescence signal of acridine orange. This data indicated that synaptic vesicles retained their proton gradient in the presence of D-mannose-coated γ-Fe2O3 nanoparticles. Uncoated γ-Fe2O3 nanoparticles also did not change synaptic vesicle acidification (data not shown). It

• spectrofluorimeter at 528 nm (excitation) and 551 nm (emission) wavelengths (slit bands 5 nm each). Measurements of synaptic vesicle acidification in synaptosomes The acidification of synaptic vesicles in synaptosomes was monitored by using acridine orange as described in [38][39]. Fluorescence changes were measured